prs314 ura3 vector  (Addgene inc)

Journal: iScience

Article Title: Grammar rules and exceptions for the language of transcriptional activation domains

doi: 10.1016/j.isci.2024.111057

Figure Lengend Snippet:

Article Snippet: Plasmid pRS314 , Addgene , Addgene Plasmid # 11004.

Techniques: Sequencing, Recombinant, Plasmid Preparation, Software

Journal: Autophagy

Article Title: Morphometric analysis of autophagy-related structures in Saccharomyces cerevisiae

doi: 10.1080/15548627.2017.1384888

Figure Lengend Snippet: Yeast strains used in this study.

Article Snippet: Ampicillin (Wako Pure Chemical Industries, 016–23301) is added to the LB medium at a final concentration of 100 μg/mL. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Name Properties Marker Reference pYEX-BX 2μ plasmid for expression of a protein from the CUP1 promoter URA3 Clontech pRS314 Centromeric plasmid TRP1 17 pYEX-BX[ APE1 ] 2μ plasmid for expression of prApe1 from the CUP1 promoter URA3 7 pRS314[ GFP- ATG8 ] Centromeric plasmid for expression of GFP-Atg8 from the ATG8 promoter TRP1 7 Open in a separate window Plasmids used in this study.

Techniques:

Journal: Autophagy

Article Title: Morphometric analysis of autophagy-related structures in Saccharomyces cerevisiae

doi: 10.1080/15548627.2017.1384888

Figure Lengend Snippet: Plasmids used in this study.

Article Snippet: Ampicillin (Wako Pure Chemical Industries, 016–23301) is added to the LB medium at a final concentration of 100 μg/mL. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Name Properties Marker Reference pYEX-BX 2μ plasmid for expression of a protein from the CUP1 promoter URA3 Clontech pRS314 Centromeric plasmid TRP1 17 pYEX-BX[ APE1 ] 2μ plasmid for expression of prApe1 from the CUP1 promoter URA3 7 pRS314[ GFP- ATG8 ] Centromeric plasmid for expression of GFP-Atg8 from the ATG8 promoter TRP1 7 Open in a separate window Plasmids used in this study.

Techniques: Marker, Plasmid Preparation, Expressing

Journal: Autophagy

Article Title: Morphometric analysis of autophagy-related structures in Saccharomyces cerevisiae

doi: 10.1080/15548627.2017.1384888

Figure Lengend Snippet: Schematic diagram for measurement of the length of GFP-Atg8-labeled structures by the manual procedure. Details are described in Section 3.2 in Methods.

Article Snippet: Ampicillin (Wako Pure Chemical Industries, 016–23301) is added to the LB medium at a final concentration of 100 μg/mL. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Name Properties Marker Reference pYEX-BX 2μ plasmid for expression of a protein from the CUP1 promoter URA3 Clontech pRS314 Centromeric plasmid TRP1 17 pYEX-BX[ APE1 ] 2μ plasmid for expression of prApe1 from the CUP1 promoter URA3 7 pRS314[ GFP- ATG8 ] Centromeric plasmid for expression of GFP-Atg8 from the ATG8 promoter TRP1 7 Open in a separate window Plasmids used in this study.

Techniques: Labeling

Journal: Autophagy

Article Title: Morphometric analysis of autophagy-related structures in Saccharomyces cerevisiae

doi: 10.1080/15548627.2017.1384888

Figure Lengend Snippet: Analysis of data obtained by Qautas. (A) Box plot using raw morphological parameters without classification by machine learning. The lengths of GFP-Atg8-labeled structures in wild-type (N = 145), atg1D211A (N = 144), and atg1K54A (N = 207) cells were extracted with ImageJ and plotted. (B) Perimeter/2 of elongated structures of wild-type (N = 53), atg1D211A (N = 57), and atg1K54A (N = 61) cells classified by machine learning. (C) Major axis length of elongated structures. *P < 0.05, **P < 0.01, N.S. not significant (Mann-Whitney U test with Bonferroni correction).

Article Snippet: Ampicillin (Wako Pure Chemical Industries, 016–23301) is added to the LB medium at a final concentration of 100 μg/mL. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Name Properties Marker Reference pYEX-BX 2μ plasmid for expression of a protein from the CUP1 promoter URA3 Clontech pRS314 Centromeric plasmid TRP1 17 pYEX-BX[ APE1 ] 2μ plasmid for expression of prApe1 from the CUP1 promoter URA3 7 pRS314[ GFP- ATG8 ] Centromeric plasmid for expression of GFP-Atg8 from the ATG8 promoter TRP1 7 Open in a separate window Plasmids used in this study.

Techniques: Labeling, MANN-WHITNEY

Journal: Autophagy

Article Title: Morphometric analysis of autophagy-related structures in Saccharomyces cerevisiae

doi: 10.1080/15548627.2017.1384888

Figure Lengend Snippet: Yeast strains used in this study.

Article Snippet: Ampicillin (Wako Pure Chemical Industries, 016–23301) is added to the LB medium at a final concentration of 100 μg/mL. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Name Properties Marker Reference pYEX-BX 2μ plasmid for expression of a protein from the CUP1 promoter URA3 Clontech pRS314 Centromeric plasmid TRP1 17 pYEX-BX[ APE1 ] 2μ plasmid for expression of prApe1 from the CUP1 promoter URA3 7 pRS314[ GFP- ATG8 ] Centromeric plasmid for expression of GFP-Atg8 from the ATG8 promoter TRP1 7 Open in a separate window Plasmids used in this study.

Techniques:

Journal: Autophagy

Article Title: Morphometric analysis of autophagy-related structures in Saccharomyces cerevisiae

doi: 10.1080/15548627.2017.1384888

Figure Lengend Snippet: Plasmids used in this study.

Article Snippet: Ampicillin (Wako Pure Chemical Industries, 016–23301) is added to the LB medium at a final concentration of 100 μg/mL. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Name Properties Marker Reference pYEX-BX 2μ plasmid for expression of a protein from the CUP1 promoter URA3 Clontech pRS314 Centromeric plasmid TRP1 17 pYEX-BX[ APE1 ] 2μ plasmid for expression of prApe1 from the CUP1 promoter URA3 7 pRS314[ GFP- ATG8 ] Centromeric plasmid for expression of GFP-Atg8 from the ATG8 promoter TRP1 7 Open in a separate window Plasmids used in this study.

Techniques: Marker, Plasmid Preparation, Expressing